Abundant and constant expression of uncoupling protein 2 in the liver of red sea bream Pagrus major.

نویسندگان

  • Xu-Fang Liang
  • Hiroshi Y Ogata
  • Hiromi Oku
  • Jianwen Chen
  • Fen Hwang
چکیده

Four overlapping cDNA fragments encoding a partial sequence for uncoupling protein 2 (UCP2) were amplified by PCR using degenerate primers from the liver of a marine teleost fish, red sea bream (Pagrus major). The partial sequence was 674 bp long, encoding 224 amino acids. The deduced amino acid sequence from the cDNA partial sequence contained the signature motifs for mitochondrial transporter protein and revealed positional identity higher than 72.8% with UCP2 from mammals. The fish UCP2 gene was highly expressed in the liver but almost undetectable in the visceral mesenteric adipose tissue. Using beta-actin as control, the UCP2 mRNA level was determined to be at least 20-fold higher in the liver than in the visceral mesenteric adipose tissues. Neither 48 h starvation nor high lipid diet had any significant effect on liver UCP2 gene expression, indicating that the abundant UCP2 gene expression was stable and might have some basic function in a fish liver that always contains high lipid content. The striking contrast of UCP2 gene expression in the two fish fat-depot organs is consistent with their large differences in oxidative capacity. We suggest that the fish liver may adapt to a constantly high fat deposit by maintaining high UCP2 expression to constrain reactive oxygen species (ROS) production and protect hepatocytes from apoptosis.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Molecular characterization of lipoprotein lipase, hepatic lipase and pancreatic lipase genes: effects of fasting and refeeding on their gene expression in red sea bream Pagrus major.

To investigate the nutritional regulation of lipid metabolism in fish, molecular characterization of lipases was conducted in red sea bream Pagrus major, and the effects of fasting and refeeding on their gene expression was examined. Together with data from a previous study, a total of four lipase genes were identified and characterized as lipoprotein lipase (LPL), hepatic lipase (HL) and pancr...

متن کامل

Whole-Genome Sequence of the Fish Virulent Strain Streptococcus iniae IUSA-1, Isolated from Gilthead Sea Bream (Sparus aurata) and Red Porgy (Pagrus pagrus)

Streptococcus iniae is a major fish pathogen that produces invasive infections that result in economic losses in aquaculture. In this study, the draft genome sequence of Streptococcus iniae strain IUSA-1, isolated from a natural outbreak affecting gilthead sea bream (Sparus aurata) and red porgy (Pagrus pagrus), is presented.

متن کامل

Exposure of eggs to solar UV-B leads to reduced hatching rates in two sparid fishes, red sea bream Pagrus major and black sea bream Acanthopagrus schlegeli.

Hatching success was examined under exposure to solar ultraviolet radiation (UVR) using filters to give three different light conditions [C1: UV-B, UV-A and photosynthetically active radiation (PAR), C2: UV-A and PAR, C3: PAR] in red Pagrus major and black Acanthopagrus schlegeli sea bream. Hatching rate of both species was reduced by an exposure over a 2 day period to UVR and was not significa...

متن کامل

Kinetic analysis of a chitinase from red sea bream, Pagrus major.

Kinetic analysis was done on the 46-kDa chitinase (EC 3.2.1.14) purified from the stomach of red sea bream, Pagrus major, using glycolchitin and N-acetylchitooligosaccharides (GlcNAc(n), n=2-6) as substrates. High activity was observed at two pHs, such as 2.5 and 9.0, toward glycolchitin as seen in other insect chitinases, and also at both pH 2.5 and 5.0 even toward a short substrate, N-acetylc...

متن کامل

Disinfection of Gilthead Sea Bream (Sparus aurata), Red Porgy (Pagrus pagrus), and Common Dentex (Dentex dentex) Eggs from Sparidae with Different Disinfectants

The aim of this study is to determine suitable conditions for egg disinfection of gilthead sea bream (Sparus aurata), red porgy (Pagrus pagrus), and common dentex (Dentex dentex) with glutaraldehyde, hydrogen peroxide and iodine. The eggs were disinfected with 200 ppm glutaraldehyde (T= 2, 4, 8, 16 min), 300 ppm hydrogen peroxide and iodine (T= 5, 10, 15, 20 min) at four different contact times...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

دوره 136 3  شماره 

صفحات  -

تاریخ انتشار 2003